Tufts Core Facility introduces Next Generation DNA Sequencing, also known as High-Throughput DNA Sequencing or Deep Sequencing. The HiSeq2000 system can generate highly accurate results in under a week for discoveries in genomics, epigenomics, gene expression analysis, and protein-nucleic acid interactions. The Roche 454 GS FLX can provide read lengths in excess of 500 bases for unparalleled analyses into metagenomics.

The HiSeq2000 system is powered by Illumina Sequencing Technology, which uses a massively parallel sequencing-by-synthesis approach to generate billions of bases of high-quality DNA sequence per run.

Applications include:

• Whole Genome Sequencing
• De Novo Sequencing, Resequencing
• Deep Sequencing of Microbiomes
• Chromatin IP analysis (ChIP-Seq)
• Transcriptome Analysis (RNA-Seq)
• Small RNA Identification and Quantitation
• DNA Methylation analysis
• Metagenomics

Currently we offer either 50 or 100 nucleotide single or paired-end (50x2, 100x2) reads. 454 GS FLX services are offered for full plates only. All sequence data, including reads mapped to a reference genome, will be available for download from our FTP site. Sequence data is archived on our server for one year. Additional bioinformatics support is available free to all Tufts users. We also offer bioinformatics consultations and support to outside institutions on a per-case basis.

All samples will be entered into a queue on receipt, and then scheduled for the next available run. At current levels, your samples will be run within 2-3 weeks. Once your run has started, turnaround time will be about a week.